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Mitochondrial aquaporin-8 knockdown in human hepatoma HepG2 cells causes ROS-induced mitochondrial depolarization and loss of viability
For a 5mg vial requiring 5ml total, the injection process should take 4560 seconds
After 24 h, cells were washed twice with cold PBS and lysed in immunoprecipitation buffer (50 mM Tris-Cl, 150 mM NaCl, 5 mM EDTA, and 1% NP-40, pH 7.8) containing 1 protease inhibitor cocktail (Sigma) on ice for 1 h
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