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A previous CSP analysis using FOXO4 FHD titration of 15 N-labeled p53 TAD showed that significant perturbations two standard deviations above average were mostly observed in residues within the p53 TAD2 region 38

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Then, cells were washed and stained in Triton 0.1% containing DRP1 antibody (Abcam ab184247) diluted 1:100