Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml 1 ) antibodies, in RPMI medium supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1) in the presence of IL-12 (3 ng ml 1 , Peprotech) (T H 1 conditions)
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Sections were scored based on the degree of colonic epithelial hypoxia as follows: 0 = no hypoxia, 1 = mild focal hypoxia, 2 = moderate multifocal hypoxia, and 3 = severe diffuse hypoxia
GHK-Cu has been shown to support nervous system recovery , making it useful for neurological healing and cognitive function