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It represents a next-generation incretin-based compound of significant interest in metabolic disease research, with studied effects on energy homeostasis, insulin secretion, and lipid metabolism in preclinical models

After 30 min incubation at room temperature, the treated lysates were further divided into smaller aliquots (50 l), which were then heated at different temperatures for 3 min on a Veriti thermal cycler (Applied Biosystems) followed by cooling for 3 min to room temperature