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doi: 10.1002/jbmr.1898 9 YangLYangJPanTZhongX

The cycling mix containing 20 M resazurin, 10 M flavin mononucleotide, 10 mM nicotinamide, 0.1% BSA in 100 mM sodium phosphate buffer, 11 g ml 1 diaphorase, 1 mM MgCl 2 , 31 mM D-glucose phosphate (G6P), and 0.03 g ml 1 glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides (G6PD, Sigma) was made freshly, and -NAD phosphate (-NADP) hydrate was used for the standard curve