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Slowly depress the plunger to deliver the medication at a steady pace about 1 ml every 10 seconds is a good guideline for minimizing discomfort

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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h

That doesn't mean people never report weight changes