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Others use low dead-volume syringes designed to minimize this waste
Rapamycin treatment of infected cells alone does not affect a wild-type Cryptosporidium infection in vitro (Fig S6A), however the addition of rapamycin to an infection with this transgenic strain abolished the development of male and female parasites, and skewed asexual parasites towards smaller, earlier stages of development (Fig 4H-I)
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