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This is accurate if the concentration is 10,000mcg/mL
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Z = N i / (1 + 10 (pK ai pH) ) N j / (1 + 10 (pH pK aj ) ) N i = Number of positively charged groups N j = Number of negatively charged groups pK ai , pK aj = pK a values of the ionizable groups The calculator employs pKa values from Lehningers Principles of Biochemistry for maximum accuracy
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