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Frozen slices 12 mm thick were blocked with PBS + 5% NGS, labeled with a Rat Monoclonal Antibody for CD54eBioKAT-1from eBioscience, Invitrogen diluted 1:200 in blocking buffer, then with an Alexa 488 Goat anti-Rat IgG secondary antibody diluted 1:500 in blocking buffer and counterstained with DAPI, and mounted in Vectashield mounting media for fluorescence
However, once a multi-dose pen is first used, the in-use period (discussed in the next section) takes precedence over the printed expiration date
One of the most surprising findings was that the lowering in Afib risk remained even among patients who gained weight while taking GLP-1 medications
Once in use, the pen can be kept at room temperature (below 30C) or in the refrigerator for up to six weeks
