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Fluorescence in situ hybridization (FISH) FISH was used to quantify cell type-specific Glp1r expression in the VTA of drug-nave, wild type Long Evans rats ( n =3 rats) and verify cell-type specific GCaMP8f expression in transgenic rats used in the fiber photometry experiments ( n= 4 rats
After cleaning the skin, whitening injection is prepared with required dosages of vials and ampoules under clinical conditions
The concentrations of reduced (GSH) and oxidized (GSSG) forms of glutathione were measured using an HPLC system (Thermo Fisher Ultimate 3000) equipped with a YMC-Pack ODS-A column (5 m, 120 , 4.6 150 mm
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