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Then, tissue sections were incubated with secondary antibody biotinylated anti-mouse IgG (1:250 dilution, BA-9200, Vector Laboratories, California, United States) and biotinylated anti-rabbit IgG (1:250 dilution, BA-1000, Vector Laboratories, California, United States) at room temperature for 1 h, followed by the Vectastain Elite ABC reagent (Vector lab) for 30 min
Unlike the C-terminal amide of Melanotan II, the free carboxylate here is titratable and carries an additional negative charge at neutral pH, so record buffer pH alongside concentration when comparing the two molecules in the same assay
Campbell, CA: VIVUS LLC
Mortality and major adverse cardiovascular events after glucagon-like peptide-1 receptor agonist initiation in patients with immune-mediated inflammatory diseases and type 2 diabetes: a population-based study
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