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GLP-1, binds to GLP-1R mostly in an -helix conformation with a large surface of contact with both the extra-cellular domain and the seven transmembrane domain 27,30

Peptides can be naturally occurring , synthetically manufactured , or structurally modified and the distinction matters, especially for drug testing, clinical use, and performance enhancement

Therefore, the optimized method was considered adequate for sample preparation and was further subjected to method validation

The SUD cases in our study were identified using standardized clinical condition codes available in the All of Us Controlled Tier dataset