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Refrigerate water for at least 2 hours before reconstitution
The development of transporter-utilizing (pro)drugs and nanocarriers for improved brain delivery begins from the understanding whether the poor efficacy of the drug is caused by low extent of the brain permeation and inadequate concentrations of the drug at target site or due to other reasons
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The third aliquot directly measures the combined pool of GSH and GSSG using previously published protocol with Ellmans reagent as a reporter
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