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In B cells, ROS are instrumental in regulating activation 31,32 , but the contribution to B cell subsets and functions of mitochondrial ROS (mtROS), which are generated principally within mitochondria, is poorly understood

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The protein precipitate was pelleted by centrifugation, the dCMP/[ 3 H]dCMP formed was isolated by ion exchange chromatography on Dowex-50 W columns, and the amount of radioactivity was quantified by liquid scintillation counter 33

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