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The NHS recommends maintaining ambient temperatures of at least 18C, using hot water bottles or heated blankets when resting, and ensuring adequate hydration with warm beverages throughout the day

Effects of moderate and subsequent progressive weight loss on metabolic function and adipose tissue biology in humans with obesity

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Quantitative real-time polymerase chain reaction (qRT-PCR) In accordance with previous studies [36], we identified 10 mRNAs for real-time fluorescent quantitative PCR (qRT-PCR) verification (Additional file 1)