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Libraries were prepared using adapter-modified PCR and sequenced on a HiSeq X Ten platform (2 150 bp PE

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Conversely, empty capsids produced from the cell culture infected at 34C (empty capsid B, Scheme 1) co-eluted with infectious CVA21 virions and required either further optimization of GSH chromatography parameters or development of a polishing chromatography step to clear residual empty capsids