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Then, we used 100 ng of cDNA for the reaction mixture
SQOR catalyzes the first and rate-limiting step in mitochondrial H 2 S oxidation by transferring electrons from sulfide to ubiquinone (CoQ 10 ), forming ubiquinol (CoQ 10 H 2 ) and a persulfide intermediate, which is further metabolized by downstream enzymes in the sulfide oxidation pathway 46,47
In contrast, sterile water is often used for one-time injections or procedures where additives could be problematic
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