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After 24 h, the medium was carefully removed and 50 l serum-free medium and 50 l MTT solution (Cayman Chemical, 21795) were added per well

Introduction Herpes simplex virus type-1 (HSV-1) is an enveloped double-stranded DNA virus belonging to the Herpesviridae family, that has a genome of approximately 152 kbp encoding more than 80 different open reading frames (ORFs

Food and Drug Administration for any medical use

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