The people who found closer to 50% in their urine probably had a slight vitamin B12 deficiency, and some of the vitamin was stored for later use
Women of reproductive age and those with heavy menstrual periods are at particular risk
The resin was then collected by centrifugation at 500 g for 10 min and washed in gravity flow column (Sangon Biotech) with five column volumes of buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl2, 1 mM MnCl2, 25 M TCEP, 0.1% (w/v) LMNG, 0.02% (w/v) CHS, 2 M GLP-1 or LY3502970 and/or 10 M compound 2, followed by washing with fifteen column volumes of buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl2, 1 mM MnCl2, 25 M TCEP, 0.03% (w/v) LMNG, 0.01% (w/v) glyco-diosgenin (GDN, Anatrace), 0.008% (w/v) CHS, 2 M GLP-1 or LY3502970 and/or 10 M compound 2
GLP-1