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Total RNA was reverse transcribed into cDNA by the High-Capacity cDNA Reverse Transcription Kit (4368814, Thermo Fisher)

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There is few clinical evidence of the effect of agomelatine in reducing overall depressive symptoms: results show agomelatine's higher response and lower remission rate compared to placebo (96, 97) and its similar efficacy compared to other antidepressants (i.e., paroxetine, fluoxetine, sertraline, escitalopram, and venlafaxine) (97, 98)

Additionally, the semaglutide 2.4-mg arm experienced significant reductions in systolic blood pressure vs the placebo arm across these trials ( P .0016)