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We did not include pediatric patients or geriatric patients older than 70 years, and our cohort was fairly demographically homogeneous, so it is unclear whether our findings are generalizable to various groups

Briefly, cohorts of 3050 adult flies (approximately 50 mg of fresh tissue) were collected in Eppendorf tubes containing and transferred to 500 ul ice-cold 50 g/l perchloric acid solution containing 0.2 M boric acid and 10 uM gamma-Glu-Glu on ice

The process differs by institution, but the American Society for Metabolic and Bariatric Surgery offers guidelines

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