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Draw to the line between 12 and 13 units
Spotting the Signs of Contamination Sometimes, despite your best efforts, contamination can occur
Isolated hippocampus was homogenized in ice-cold nondetergent hypotonic buffer (10 mM HEPES [pH 7.9], 1.5 mM MgCl 2 , 10 mM KCl, 100 mM DTT, protease and phosphatase inhibitor cocktail)
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