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Real-time PCR (RT-PCR) was performed on a Mastercycler Nexus Gradient GSX1 thermal cycler (Eppendorf, Hamburg, Germany) using Phanta Max Super-Fidelity DNA Polymerase
Notably, these studies found that simple endothelial cell cultures did not show the same angiogenic response, suggesting that BPC-157 may act as a context-dependent regulator of vascular signaling rather than a direct angiogenic stimulus
Nevertheless, current GSH-activated prodrugs frequently face challenges in achieving selective activation within heterogeneous tumor microenvironments
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