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The reconstituted solution will not be colourless: expect a blue to blue-violet tint from the Cu(II) centre of the GHK-Cu component, and treat a colourless or green-tinged solution as evidence the copper complex has been disrupted rather than merely diluted, with visible absorption around 500 to 600 nm as the standard check

Thus, oxidative stress in the vasculature will stimulate ADMA production and/or inhibit ADMA degradation, leading to eNOS inhibition by this endogenous molecule