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Detailed Protocol

For instance, co-culturing cardiomyocytes, ECs, smooth muscle cells, and cardiac fibroblasts derived from human-induced pluripotent stem cells (iPSCs) employing the forced floating method using ultra-low attachment plates have been shown to result in the formation of cardiac spheroids [252]

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These elevated ROS levels damage oxidative DNA, prominently affecting the TP53 tumor suppressor gene