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In parallel experiments, whole brains from two rats were rapidly removed and placed in ice-cold isolation buffer
Time-lapse imaging Videos of freshly isolated mouse tubules stained with 50 nM SYTOX Green nucleic acid stain (Life Technologies) in the presence or absence of 150 nM Biotracker 609 Red Ca 2+ AM dye (Merck Millipore, 5.04297.0001) or 200 nM MitoTracker Red FM (Invitrogen, M22425) were obtained using an oil-immersion 63/0.3 EC Plan Neofluar objective
BPC-157 and TB-500 combined
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