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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)
The most commonly reported side effects are increased appetite (consistent with ghrelin receptor activation), mild water retention and transient edema in the first 1-2 weeks, and occasional muscle cramping
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Kim TH, Jiang HH, Lim SM, Youn YS, Choi KY, Lee S, et al
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