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WAT mitochondria isolation To isolate mitochondria, fresh gWAT was minced then homogenized using a drill-operated Teflon pestle in ice-cold MSHE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EDTA, pH 7.2) containing 0.5% FA-free BSA

This study is centered around the effects of HMB supplementation on oxygen capacity for endurance athletes, which found that blood glucose was significantly greater during HMB use and concludes that HMB supplementation may have positive effects on performance[12]

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Nutraceuticals are regulated by the FDA as a dietary supplement