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Immunofluorescence microscopy For the detection of markers of functional enterocytes (CFTR and FABP1, 1:100) or tight junction proteins (ZO-1, 1:100), fully differentiated organoid or organoid-derived monolayer cultures grown in 8-well chambers or 24-well transwells were fixed using 4% paraformaldehyde (PFA), permeabilized with 0.1% Triton X-100, and blocked with 3% BSA
Monitor for interactions: While peptides and GLP-1s work through different mechanisms, individual responses vary
doi: 10.1038/ijo.2016.67
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