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Lancet Diabetes Endocrinol 2019;7:715725

The extent of oxidative burst, as estimated by induction of a GST gene, was not weakened by the absence of light (Zeier et al., 2004)

10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation