They also noted that subjects in this study were either overweight, obese or had type 2 diabetes, the latter of which is an established risk factor of NAION, with others including heart disease, history of heart attack, high blood pressure and sleep apnea
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These include sudden onset of severe leg pain, particularly if unilateral and associated with swelling or warmth (which could suggest deep vein thrombosis), progressive weakness or numbness in the legs, loss of bladder or bowel control, or symptoms accompanied by fever or signs of infection
The most straightforward should be the reaction with acid chlorides since they are the most reactive carboxylic acid derivatives, and amines are good nucleophiles too