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Polar compounds were separated via an HILIC column (Agilent ZORBAX HILIC Plus, 3.0 mm 100 mm, 1.8 m), and lipid compounds were separated via a C18 column (Thermo Hypersil Gold C18, 2.1 mm 100 mm, 1.9 m)

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Despite this reduction, the proportion of LBM relative to total BW increased by 3% points, and the lean-to-FM ratio improved significantlyparticularly among individuals who achieved 15% weight loss [13, 78]

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