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Cells were fixed with 2% paraformaldehyde (15 min), permeabilized with 0.1% Triton X-100 (10 min) and blocked with 2% BSA (1 h)
Gao et al., 2013
Prevalence of Hyperhomocysteinaemia and Associated Factors among Ethiopian Adult Population in a 2015 National Survey
(3) relative humidity: sufficient air humidity has a positive effect on the growth and quality of spinach as dry and heat weather cause bolting of spinach, Environmental factors such as long day length and high temperature, dense cultivation, low soil fertility and insufficient irrigation are among the most important factors for the bolting in spinach, and nutrient composition in hydroponic cultivation 3
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