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Not everyone benefits from split dosing
Additionally, a randomized, double-blind, placebo-controlled trial in 2,588 healthy older adults with mild hyperhomocysteinemia found that daily supplementation with 500 g of vitamin B 12 and 400 g of folic acid for two years reduced blood homocysteine concentrations but had no benefit on depressive symptoms in those with or without depressive symptoms at baseline (110)
You can assess the effectiveness of treatment done by using vitamin b12 injection for cats through observable improvements or veterinary testing
& Jensen, J
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