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Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed using a QuantiTect SYBR-Green RT-PCR kit (Qiagen, Venlo, The Netherlands) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA)
Age- and sex-dependent profiles of APP fragments and key secretases align with changes in despair-like behavior and cognition in young APPSwe/Ind mice
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It does not directly enhance fat oxidation the way levocarnitine does, but it addresses the B12 deficiency risk that increases with reduced food intake on GLP-1 therapy
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