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A range of concentrations from 100 M to 5 mM was tested, and it was found that concentrations between two mM and five mM provided the most accurate results for GPx activity measurement
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With doses of 10 nmol/kg and higher, rats exhibited not only a lack of activity, but also notable changes in facial expressions associated with pain and nausea including orbital tightening and nose/cheek flattening compared to rats treated with vehicle alone
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