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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)
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The transcriptional profiles of Bmp4 in the Hedgehog pathway showed downregulation by 4.53 folds, with Lep in the Insulin pathway being downregulated by 5.26 folds
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