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electrochemiluminescence detection of each MSD SULFO-TAG was captured and quantified using SECTOR Imager instrumentation (MSD, Gaithersburg, MD, USA), according to manufacturers protocols as reported previously ( The raw data were analyzed using the Discovery Workbench 4.0 software (MSD) by fitting signal from the control calibration curves to a four-parameter logistic model and then back-fitting the electrochemiluminescence signal from each sample to calculate the unknown concentration
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Aim for seven to nine hours nightly, maintaining consistent sleep and wake times when possible
Proceedings of the National Academy of Sciences of the United States of America 106, 1708917094, doi: 10.1073/pnas.0904649106 (2009)
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