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Disruption of network synchrony and cognitive dysfunction after traumatic brain injury
The assay was conducted in the wells of 96-well black plastic microplates, in which 20 L of enzyme solution and 60 L of bile salt were mixed together, and then 20 L of substrate was added, with mixing, at the start of the reaction, which proceeded at room temperature for up to 30 min, with measurement at various intermediate time points
Joshi SD, Dixit SR, Kirankumar MN, et al
HPLC-grade water was produced using a Milli-Q ultrapure water purification system (Bedford, MA, United States)
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