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Briefly, flash-frozen tissue samples were homogenized in ice-cold PBS containing 2 mM EDTA (1 ml PBS/EDTA per 10 mg tissue) using a Dounce homogenizer
567 ToogoodPLClauwDJPhadkeSHoffmanD
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The model of CTR: rAmy (CT-like, PDB: 7TYI) was used as the initial template for the CTR:Cagri/CagriBB complex in the CT-like conformation by replacement of the peptide
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