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Samples were resolved on 12% or 10-20% SDS-PAGE gels (Invitrogen) and analyzed by standard immunoblotting protocol

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The BENDIP study demonstrated that neuropathic symptoms, measured using the NIS as the primary endpoint, improved after 6 weeks of treatment with benfotiamine at a dose of 300 mg twice daily (no improvement was observed with 300 mg once daily) [71]

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