For the total and active GLP-1 assay, dried pellets were resuspended in RIA buffer, and for MS analysis, dried pellets were resuspended in PBS, and in each case, the protein concentration was determined using the Pierce BCA Protein Assay Kit (Prod# 23225, ThermoFisher Scientific, France)
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A8: The best time is 2030 minutes before exercise
GLP-1s reduce appetite significantly, which drives weight loss, but some of that weight loss can come from muscle, not just fat