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Q-PCR reaction mixtures contained 1 l cDNA, 1x SYBR Green Supermix (BioRad, Hercules, CA), and 0.15 M gene-specific primer sets in a total volume of 25 l
Interestingly, at the age of 47 years P20 developed extensive surgical emphysema within the subcutaneous tissues of her face and computed tomography (CT) scans of the sinuses revealed a defect of the left nasal cartilages anteriorly allowing air to track into the soft tissues
Fort 2017a
[PubMed] Bystrm J, Johansson B, Edsmyr F, et al
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