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Given the screening data indicating that mitochondria are a dominant metabolic target in response to dual SHP2/MEK inhibition across PDAC subtypes, we investigated mitochondrial behavior under MAPK pathway inhibition in more detail

Acquity UPLC CSH C18 (1.7 m, 2.1 100 mm) column (Waters Corp.) was used to separate constituent lipid species

Informed by recent studies from 2024-2025, future research should prioritize the development of disease-specific ferroptosis biomarkers, optimization of combination therapy regimens, creation of next-generation modulators with enhanced cell-type specificity and pharmacokinetic properties, and elucidation of ferroptosis crosstalk with other regulated cell death pathways (152)