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2c, 3c), animals were prepared as above, but coverslips were sealed with VALAP (1:1:1 vaseline, lanolin, paraffin), and 0.35 m z-step stacks were acquired with a Leica SP8 point scanning confocal microscope with a HC PL APO CS2 40x/1.30 numerical aperture oil objective with a zoom factor of 1 for full gonad images, or of 4 for DTC images