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Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments
Hepatic F4/80 + cells were isolated from dissociated liver samples with Anti-F4/80 MicroBeads UltraPure (mouse, Miltenyi Biotec) as per manufacturers protocol and flash-frozen in liquid nitrogen before being stored at 80C for mRNA extraction
Much like any other disease state, if one type of therapy isnt feasible due to cost, access, tolerance, or just ineffectiveness for a specific patient, then pursuing an alternate treatment strategy is a very reasonable course of action, says Maselli
BMJ Open 15: e094947
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