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The membrane was washed three times with TBST (0.1% Tween) buffer, sealed with protein-free rapid closure solution (Shanghai Baibo Biotechnology Co., Ltd.) at room temperature for 30 min, washed three times again and then incubated with the primary antibodies on a shaker at 4C overnight
Its cobalt-blue hue is unmistakable whether as a powder or infused into a serum
Table 1 Technical challenges and strategies for quality control in kidney organoids
Dogru T, Kirik A, Gurel H, Rizvi AA, Rizzo M, Sonmez A
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