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coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)
Both 1/ values were similar to the corresponding apparent catalytic efficiencies and decreased for K105X mutants by up to one or two orders of magnitude (Fig
Navas LE et al
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