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Most protocols run eight to twelve weeks, then stop for a reassessment
All primary antibody incubations (1:1000 in TBST, overnight at 4 C) were followed by incubation with a secondary HRP-conjugated antibody (1:5000 in TBST, 1 h at room temperature), and the signals were visualized using a chemiluminescent HRP substrate on a chemiluminescence imaging system (CHAMPCHEMI TM Chemiluminescent Imaging and Analysis System)
Permission to use the MoCA was provided by Dr Ziead Nasreddine
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