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Tissue culture media supernatants from the final 24 hours of culture were collected and assayed by Amplex Red Glucose/Glucose Oxidase Assay Kit (Life Technologies, Gaithersburg, MD, USA), Lactate Assay kit (Sigma-Aldrich) and Rat Ultrasensitive Insulin ELISA (Mercodia, Uppsala, Sweden) to measure total glucose, lactate and insulin respectively

The difference in E of roGFP2-iL and GRX1-roGFP2-iL suggests that N-terminal fusion of GRX1 influences the thermodynamic properties of the C147C204 disulfide

Oncogenic KRAS regulates amino acid homeostasis and asparagine biosynthesis via ATF4 and alters sensitivity to L-asparaginase

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